INVESTIGATION OF PHYTOCHEMICAL AND ANTIOXIDANT ACTIVITY OF ACACIA NILOTICA FROM JHELUM, PUNJAB PAKISTAN
Abstract
The phytochemical and antioxidant activities of leaf and bark of Acacia nilotica (Linn.) wild of family Fabaceae were evaluated to study ethno-pharmacological importance. In phytochemical investigation qualitative and quantitative tests were performed. Qualitative tests were performed on ethanolic extract of leaves and bark of Acacia nilotica. Phytochemical analysis showed that most of the phytochemical contents were present in Acacia nilotica leaves and bark. Trite
rpenoids, Sterols, Proteins, Carbohydrate, Tannins, Glycosides were present in better quantity rather than the Alkaloids were only detected in leaves. Quantitative tests were performed to know the quantity of Alkaloids and Tannins in the bark and leaves of Acacia nilotica. 7.8% Alkaloids were present in leaves and Alkaloids were not detected in bark. 2.64% Tannins were present in leaves and 6.09% Tannins were present in bark of Acacia nilotica. Antioxidant effect was investigated by using two methods and maximum result of DPPH was detected by A. nilotica leaves 0.919±0.03 mm in ethanolic extract of leaves at 1000 concentration. Maximum result of DPPH was detected by A. nilotica bark 0.694±0.06 mm in ethanolic extract of bark at 1000 concentration. Total phenolic content of A. nilotica leaf extract of ethanol was 0.806±0.08 mm and Total phenolic content of A. nilotica bark extract of ethanol of 0.803±0.11 mm showed maximum value at 1000 concentration.